Hi Elaine, I've not experimented much with the tree lab but here's how I applied texture and transparency maps to the standard Aspen tree. The maps were 600 pixels square. First I loaded the Aspen tree. Switching to the Texture there is a basic Layers List shader for the tree. Note that there's a layer for 'leaf'. Load your maps in this layer, the texture in the Colour channel and using a Multichannel mixer to get the transparency. HTH Mark