Without seeing your settings Sharky, here's my thoughts. I'm sure you have your trans map in the opacity slot in studio, which transfers into the Alpha channel in reality. Is your alpha channel set to 1.0?

The only other thing I can think of is that your black may not be absolute. Again, that's really a guess from this side of the screen. I have learned recently that the trans maps are absolutely too large in png size wise, so the 'ol grayscale jpg fits the bill.

Just so we're on the same page, you've got your colors coordinated so black=fully transparent and white=fully opaque, right?

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