tjames opened this issue on Dec 10, 2002 ยท 12 posts
tjames posted Wed, 11 December 2002 at 1:31 PM
The turn was meant to be quick on a huh? I was thinking the machine reading off a-a-a-g-g-c-c-c some combinations mean something, some nonsense, some their actions masked by other combinations. On the thousands of possible combinations like if you have an allele that says produce this protein and its activity is countered by another that says bond a sequence that makes that protien useless. Not every combination is expressed. Anyways back in the seventies when I went through that part of my rotation every part of the genetic lab was so "primative" most of the methods are probably historical. I know what a sequencer does and can imagine the young man's boredom because I used to sit watching bubbles flow though the tubes of an autoanalyzer, watching the printout of sugar, bun, creatinine, sgot, sgpt,ldh and the mind did wander. All you had to do was keep up the fluids and replace the sample tray when it ran out....occassionally checking a standard or a control to make sure the quality control was in. Do you see where I'm going. If I can draw an allegory between each bubble being a specimen, a person, people, running through the tubes and a city...cars traffic. Anyways I took genetics back in 1977. Every once in a while my mind fades back to the day...but back then I had my trusty bowmar brain and a wang to do all the calculations in the lab. There were no PC's and I used punch cards to put results in the computer which took up an entire room and handled millions of calculations per minute. (Apollo 13).